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Characterisation of anti-KRT14 recombinant antibodies

KRT14 (Keratin 14) is an intermediate filament protein highly abundant in epithelial cells. The internal histology lab at INRAE St Gilles has identified two recombinant antibodies that recognise KRT14 in livestock species.

The internal histology laboratory in St Gilles tested four recombinant anti-KRT14 antibodies on cells and tissues from cows, goats and sows.
- One antibody (CST 74956S) was validated in all three species.
- One antibody (Abcam Ab119695) detects KRT14 in cow tissues (2D cell culture and mammary gland sections) and in sows, but not in goats.
- One antibody (AbAb RCK107) detected bovine KRT14 only in 2D cell culture, and the staining was partial.
- One antibody (AbAb 2GA) did not produce a signal under the conditions tested.

Legend: staining with anti-KRT14 CST 74956S (Recombinant monoclonal) on a section of bovine mammary gland © Histo lab St Gilles

Characterisation by:
Atelier histologie interne de l'INRAE de St Gilles

Recombinant antibodies tested:
AbAb 2G4
AbAb RCK107
Abcam Ab119695
CST 74956S

Materials & methods:
Download link

Related publication(s):
https://hal.science/hal-05285752

Biological materials tested

Antibodies tested

MAC-T epithelial cell culture
(cow)

Mammary gland
(cow)

Mammary gland
(goat)

Mammary gland
(sow)

AbAb 2G4

Not validated

See results

Not validated

See results

Not validated

See results

Not validated

See results

AbAb RCK107

Not validated

See results

Not validated

See results

Not validated

See results

Not validated

See results

Abcam Ab119695

Validated

See results

Validated

See results

Not validated

See results

Validated

See results

CST 74956S

Validated

See results

Validated

See results

Validated

See results

Validated

See results

Antibodies tested

Biological materials tested

AbAb 2G4

MAC-T epithelial cell culture
(cow)

Not validated

See datas

Mammary gland
(cow)

Not validated

See datas

Mammary gland
(goat)

Not validated

See datas

Mammary gland
(sow)

Not validated

See datas

AbAb RCK107

MAC-T epithelial cell culture
(cow)

Not validated

See datas

Mammary gland
(cow)

Not validated

See datas

Mammary gland
(goat)

Not validated

See datas

Mammary gland
(sow)

Not validated

See datas

Abcam Ab119695

MAC-T epithelial cell culture
(cow)

Validated

See datas

Mammary gland
(cow)

Validated

See datas

Mammary gland
(goat)

Not validated

See datas

Mammary gland
(sow)

Validated

See datas

CST 74956S

MAC-T epithelial cell culture
(cow)

Validated

See datas

Mammary gland
(cow)

Validated

See datas

Mammary gland
(goat)

Validated

See datas

Mammary gland
(sow)

Validated

See datas

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