Ki67 (Kiel, clone 67) is a nuclear marker of cell proliferation. The RHEM platform has identified three recombinant antibodies that detect Ki67 in mice.
The RHEM platform tested three anti-Ki67 recombinant antibodies on tissue sections (intestine and spleen) from mice.
The three antibodies (Ab16667, Ab279653 and MA5-14520) produced an intense, biologically relevant signal with little or no background noise.
The staining obtained with the three recombinant antibodies is similar to each other, but not comparable to that obtained with the platform's reference monoclonal antibody (less intense and more extensive signal), which is no longer commercially available. These results suggest that more specific staining could be obtained with the recombinant antibodies than with the antibody to be replaced.
Characterisation by:
Recombinant antibodies tested:
Materials & methods:|
Biological materials tested Antibodies tested | Intestine | Spleen |
|---|---|---|
Validated | Validated | |
Validated | Validated | |
Validated | Validated |
Antibodies tested |
Biological materials tested |
|---|---|
Intestine Validated | |
Spleen Validated | |
Intestine Validated | |
Spleen Validated | |
Intestine Validated | |
Spleen Validated |
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