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Sandra Wiart-Letort, Laurence Finot
Posted on Tuesday September 23, 2025
DOI: 10.60675/yfh3-8k03/sn20250923-8/short-notes 
As part of the GIS FC3R leading project to replace animal-derived antibodies by recombinant ones, we tested four recombinant antibodies to detect by immunofluorescence, keratin 14, a protein expressed by the basal epithelial cells. Two antibodies, the clones E7W6V and SP53 from respectively, Cell Signaling Tech. and Abcam, were able to recognize the target in the mammary gland of bovine and porcine species. Only the clone E7W6V recognized keratin 14 in the mammary tissue of all three species, including goats.
